A region of the cellobiohydrolase I promoter from the filamentous fungus Trichoderma reesei mediates glucose repression in Saccharomyces cerevisiae, dependent on mitochondrial activity
The upstream activating region that controls cellulose-induced expression of the glucose-repressible cellobiohydrolase I gene (UARcb1) of the filamentous fungus Trichoderma reesei shown to mediate transcription and glucose repression of a reporter gene in Saccharomyces cerevisiae, a unicellular microorganism that lacks the genes required for the utilization of cellulose. Glucose-controlled transcription mediated byUARcb1requires the products of the genesSNF1andSSN6,a protein kinase and a repressor, respectively, that regulate glucose-repressible yeast genes. Previously, it has been shown that mitochondrial function is implicated in cellobiohydrolase I gene expression inT. reeseiand this sensitivity to the metabolic state of the mitochondria was shown to be transcriptionally controlled by the 5-flanking sequence of thecbh1gene [Abrahão-Netoet al.(1995)Biochemistry 34,10456-10462]. Remarkably, transcription of the reporter gene controlled byUARcb1inS. Cerevisiae also showed a requirement for active mitochondria, suggesting that a common mechanism involving mitochondrial activity controls glucose-repressible genes in both microorganisms.

Apoio financeiro: PADCT/CNPq e FAPESP


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